The ANCHOR C. difficile PCR Kit is a specialized real-time PCR-based test designed for the precise amplification and detection of nucleic acids specific to toxigenic Clostridioides difficile, including:

◽ Detection of Toxin A (tcdA) DNA
◽ Detection of Toxin B (tcdB) DNA

Key Features

Rapid Results

Achieve efficient sample processing with PCR results within just 30 minutes*.
* Exact PCR run time may vary depending on real-time PCR instrument used.

Precise Qualitative Detection

• Identifies and detects toxigenic C. difficile-specific DNA of toxin A and toxin B genes (w/o differentiation) and the Internal Control using distinguishable fluorescent dyes across two detector channels.

Integrated Quality Monitoring

• Includes an Internal Control (IC) to verify sample extraction success and detect PCR amplification inhibition.

• Features a Positive Control with artificial DNA representing target sequences of both toxin A and toxin B genes to ensure reagent integrity and reaction performance.

• Comes with a Negative Control DNA to exclude false positive results.

Optimized for Efficiency & Flexibility

• Compatible with all ANCHOR PCR kits in the same run (harmonized temperature profiles)

• Intuitive and identical pipetting scheme for all ANCHOR PCR kits

• Works with various nucleic acid purification systems and cycler platforms for cost efficiency and operational ease

• 100-reaction kits include 4x 25-reaction Master Mix vials to reduce freeze-thaw cycles